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The results showed that the and subunits, two of the three regulatory subunits of eIF2B, were present in higher levels in the carcinoma lysates than in the nontransformed cell lysates (Figure 3E, compare lanes 4 with lanes 2, 3). Lane 1 contains purified eIF2B as a control. Similarly, subunit , the accessory subunit of the catalytic subunit , was also higher in carcinoma cell lysates (data not shown).